Last data update: 24 November 2020 04:18 CET
Plasmid name: pPLSTi531 (LMBP 1817)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Satellite tobacco necrosis virus (STNV); with insertion|
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function|
|Further information:||The plasmid construction is the same as that of pPLSTi322, the only difference is that here the AvaII of nucleotide position 531 of STNV was treated, and that the linker is orientated in opposite direction. See also pPLSTi322.
The reading frame moves up one nucleotide, and termination occurs at a new stop codon (TAG) at nucleotide position 640 of STNV, enlarging the coat protein.
Name mentioned in Van Emmelo et al. (1984) is pSTNVN531.
Other names of the plasmid are pSTPU1L5, pPLSTNVA22 and pSTNVE531.
|EMBL Accession number:||-|
|Latest sequence update:||22/09/1989|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Van Emmelo et al., Virology 136 (1984), 32-40 [PMID: 18639819]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 r-m+ (λ)|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.