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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLSTi531 (LMBP 1817)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1817.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Satellite tobacco necrosis virus (STNV); with insertion
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function
Parental clone: pPLST10
Further information: The plasmid construction is the same as that of pPLSTi322, the only difference is that here the AvaII of nucleotide position 531 of STNV was treated, and that the linker is orientated in opposite direction. See also pPLSTi322.
The reading frame moves up one nucleotide, and termination occurs at a new stop codon (TAG) at nucleotide position 640 of STNV, enlarging the coat protein.
Name mentioned in Van Emmelo et al. (1984) is pSTNVN531.
Other names of the plasmid are pSTPU1L5, pPLSTNVA22 and pSTNVE531.
EMBL Accession number: -
Latest sequence update: 22/09/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Van Emmelo et al., Virology 136 (1984), 32-40 [PMID: 18639819]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 r-m+ (λ)
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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