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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLSTi436b (LMBP 1816)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1816.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Satellite tobacco necrosis virus (STNV); with insertion
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function
Parental clone: pPLST10
Further information: A stop codon (TGA) is created with the first nucleotide of the linker.
This plasmid is derived from pPLST10. After a partial FokI digest (nucleotide position 432 of STNV), the site was filled in and the EcoRI-NcoI linker (AGAATTCCCATGGA) was inserted.
Name mentioned in the reference is pSTNVN434.
Other names of the plasmid are pSTPU1L4, pPLSTNVE434 and pSTNVF11.
EMBL Accession number: -
Latest sequence update: 22/09/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Van Emmelo et al., Virology 136 (1984), 32-40 [PMID: 18639819]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 r-m+ (λ)
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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