GREAT AT SMALL THINGS

0

LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLSTi436a (LMBP 2029)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2029.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Satellite tobacco necrosis virus (STNV); with insertion
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function
Parental clone: pPLST10
Further information: This plasmid is derived from pPLST10: after a partial FokI digest (nucleotide position 432 of STNV), the site was filled in and the NcoI-EcoRI linker (TCCATGGGAATTCT) was inserted.
The amino acids reading frame is conserved, and the coat protein contains 6 extra amino acids.
Other names of the plasmid are pSTNVN434, pPLSTNVN434 and pSTPU1l24.
EMBL Accession number: -
Latest sequence update: 22/09/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Ameloot(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 K514(λ)
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search