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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPLST10 (LMBP 2044)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pplst10.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Satellite tobacco necrosis virus (STNV)
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function
Parental clone: pPLc2820; pUR222
Further information: The plasmid was constructed as follows: the dG/dC-tailed cDNA of STNV was inserted into the unique PstI site of a modified pPLc2820: at the 3' end of the STNV gene, the PstI site is located next to the SalI site and the XbaI site in between is lost. The inserted STNV fragment is complete.
Detailed construction information can be found in Van Emmelo et al. (1984), fig. 1 p. 34.
Names mentioned in the reference are pCP403 and pSTNV403.
Other name of the plasmid is pSTPU1.
EMBL Accession number: -
Latest sequence update: 12/09/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Van Emmelo et al., Virology 136 (1984), 32-40 [PMID: 18639819]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 K514(λ)
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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