LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPICZBBAX (LMBP 6635)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results


Cloned DNA: Mouse Bcl2-associated X cDNA (Bax, BAX); α variant
Myc epitope; C-terminal
Histidine tag (His-tag); C-terminal
Promoter: Pichia pastoris alcohol oxidase 1 promoter (AOX1)
Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α)
Synthetic prokaryotic EM7 promoter
binding site:
Terminator: Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient and endonuclease A deficient strains
Pichia pastoris; integrative
Parental clone: pUC19B; pPICZB
Further information: The plasmid was created by isolating the mBax cDNA from pUC19B as a HindIII (Klenow blunted)/EcoRI fragment and cloning it into the NotI (Klenow blunted)/EcoRI-opened pPICZB vector.
The C-terminal Myc epitope and histidine tag are not expressed.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726836.1.
The nucleotide sequence of the mBax cDNA corresponds with the EMBL Nucleotide Sequence Database accession number L22472.1.
EMBL Accession number: L22472.1, view at EMBL, GenBank, DDBJ
LT726836.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 16/03/2010
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI/SphI, EcoRV and Alw44I.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr. P. Tiels(1) (2) and Prof. Dr N. Callewaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: Martinet et al., Biotechnol. Lett. 21 (1999), 821-829
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + zeocin (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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