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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPICZA-AGA1 (LMBP 8501)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Saccharomyces cerevisiae Aga1p cDNA (AGA1, GeneID 855780)
Promoter: Pichia pastoris alcohol oxidase 1 promoter (AOX1)
Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α)
Synthetic prokaryotic EM7 promoter
Ribosome
binding site:
-
Terminator: Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient and endonuclease A deficient strains
Pichia pastoris; integrative
Parental clone: pPICZA
Further information: The plasmid was constructed by cloning the S. cerevisiae Aga1p coding sequence as an XhoI/ApaI fragment into the pPICZA vector.
This plasmid is part of a Pichia Surface Display system, together with the pPIC9-AGA2 vector (LMBP 08502).
Other names of the plasmid are pPICZA AGA1, pPICZAAGA1 and pPICZA AGA1 -XhoI/-ApaI.
EMBL Accession number: -
Latest sequence update: 12/07/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Dr K. De Schutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Jacobs et al., Biotechnol. Lett. 30 (2008), 2173-2181 [PMID: 18679585] [DOI: 10.1007/s10529-008-9807-1]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + zeocin (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Plasmid name: pPICZA-AGA1 (LMBP 8501)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Saccharomyces cerevisiae Aga1p cDNA (AGA1, GeneID 855780)
Promoter: Pichia pastoris alcohol oxidase 1 promoter (AOX1)
Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α)
Synthetic prokaryotic EM7 promoter
Ribosome
binding site:
-
Terminator: Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient and endonuclease A deficient strains
Pichia pastoris; integrative
Parental clone: pPICZA
Further information: The plasmid was constructed by cloning the S. cerevisiae Aga1p coding sequence as an XhoI/ApaI fragment into the pPICZA vector.
This plasmid is part of a Pichia Surface Display system, together with the pPIC9-AGA2 vector (LMBP 08502).
Other names of the plasmid are pPICZA AGA1, pPICZAAGA1 and pPICZA AGA1 -XhoI/-ApaI.
EMBL Accession number: -
Latest sequence update: 12/07/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Dr K. De Schutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: -
Cultivation medium: LB-Lennox + zeocin (25 μg/ml)
Cultivation temperature: 37°C°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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