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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPICZA(-BstBI) (LMBP 5763)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p5763.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Myc epitope; C-terminal
Histidine tag (His-tag); C-terminal
Promoter: Pichia pastoris alcohol oxidase 1 promoter (AOX1)
Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α)
Synthetic prokaryotic EM7 promoter
Ribosome
binding site:
-
Terminator: Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient and endonuclease A deficient strains
Pichia pastoris; integrative
Parental clone: pPICZA
Further information: A unique BstBI site at the end of the AOX1 promotor region is removed by digesting the pPICZA vector with BstBI and blunting via Klenow fill-in. The blunt vector was closed via self-ligation.
EMBL Accession number: -
Latest sequence update: 16/03/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr N. Callewaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + zeocin (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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