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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPICZ-E-MALT1Cd180::GyrB-Myc-His (LMBP 5745)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, GeneID 10892); fragment
Escherichia coli DNA gyrase subunit B (gyrB); fragment
E-tag; N-terminal
Myc epitope; C-terminal
Histidine tag (His-tag); C-terminal
Promoter: Pichia pastoris alcohol oxidase 1 promoter (AOX1)
Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α)
Synthetic prokaryotic EM7 promoter
Ribosome
binding site:
-
Terminator: Pichia pastoris alcohol oxidase 1 terminator (AOX1)
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo))
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Pichia pastoris; integrative
Parental clone: pPICZA; pCAGGS-E-MALT1Cd180::GyrB
Further information: The plasmid was constructed by PCR amplifying the E-tagged human MALT1Cd180::GyrB fragment from pCAGGS-E-MALT1Cd180::GyrB and ligating it as a KpnI/ApaI fragment into the pPICZA vector.
The plasmid was created for production of recombinant human MALT1 in Pichia pastoris for in vitro studies.
It encodes for residues 334-664 of human MALT1 fused to the E. coli GyraseB dimerization domain.
The nucleotide sequence of the human MALT1 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2.
Other name of the plasmid is pPICZ-MALT1C d180 Gyr B
EMBL Accession number: AF130356.2, view at EMBL, GenBank, DDBJ
Latest sequence update: 09/04/2009
Sequence detail:
Primers used to amplify the E-tagged Malt1Cd180::GyrB fragment:

ApaI-GyrB-R:     5' ggcGGGCCCGCCTTCATAGTGGAAGTGGTCTT

KpnI-pCAGGS-E-F: 5' gaattGGTACCaattccaccatgggtgcgcc
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2).
(1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + zeocin (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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