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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPAS5 (LMBP 2094)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: ppas5.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli papA gene (papA); mutant
Promoter: Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage MS2 polymerase gene; hybrid with papA SD
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pPLc245; pPAS2
Further information: This plasmid was constructed as follows : the EcoRI-XbaI fragment of pPAS2 (containing the 7B mutant of E. coli papA gene) was inserted between the EcoRI and XbaI sites of pPLc245.
Other name of the plasmid is pPLcPAPA7B.
EMBL Accession number: -
Latest sequence update: 04/06/1993
Sequence detail:
Mutation of the E. coli papA gene:


 signal sequence

 ATG ATT AAG TCG GTT ATT GCC GGT GCG GTA GCT ATG GCA GTG GTG TCT 
 MET Ile Lys Ser Val Ile Ala Gly Ala Val Ala Met Ala Val Val Ser 

                        | mature papA
                        | 1   2   3   4   5   6   7   8   9   10
 TTT GGT GTA AAT AAT GCT GCT CCA ACT ATT CCA CAG GGG GAT CCT AAA 
 Phe Gly Val Asn Asn Ala Ala Pro Thr Ile Pro Gln Gly Asp Asn Lys 
                                                   BamHI


E. coli papA gene wt :

 signal sequence

 ATG ATT AAG TCG GTT ATT GCC GGT GCG GTA GCT ATG GCA GTG GTG TCT 
 MET Ile Lys Ser Val Ile Ala Gly Ala Val Ala Met Ala Val Val Ser 

                        | mature papA
                        | 1   2   3   4   5   6   7   8   9   10
 TTT GGT GTA AAT AAT GCT GCT CCA ACT ATT CCA CAG GGG CAG GGT AAA 
 Phe Gly Val Asn Asn Ala Ala Pro Thr Ile Pro Gln Gly Gln Gly Lys
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr L. Steidler(1) (2) and Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Steidler et al., J. Bacteriol. 175 (1993), 7639-7643 [PMID: 7902348]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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