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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pPAS2 (LMBP 2082)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: ppas2.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli papA gene (papA); mutant 7B
Promoter: -
Ribosome
binding site:
-
Terminator: Phage fd terminator
Selection marker: Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pPAS1
Further information: This plasmid was constructed by site-specific mutagenesis (Eckstein method) of the papA gene; a BamHI site is created at AA 7 of mature papA.
The plasmid contains the mutated, promoterless papA gene.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
Other name of the plasmid is pMc58PAPA7B.
EMBL Accession number: -
Latest sequence update: 04/06/1993
Sequence detail:
Mutation of the E. coli papA gene:

 signal sequence

 ATG ATT AAG TCG GTT ATT GCC GGT GCG GTA GCT ATG GCA GTG GTG TCT 
 MET Ile Lys Ser Val Ile Ala Gly Ala Val Ala Met Ala Val Val Ser 

                        | mature papA
                        | 1   2   3   4   5   6   7   8   9   10
 TTT GGT GTA AAT AAT GCT GCT CCA ACT ATT CCA CAG GGG GAT CCT AAA
 Phe Gly Val Asn Asn Ala Ala Pro Thr Ile Pro Gln Gly Asp Asn Lys 
                                                   BamHI




E. coli papA gene wt :

 signal sequence

 ATG ATT AAG TCG GTT ATT GCC GGT GCG GTA GCT ATG GCA GTG GTG TCT 
 MET Ile Lys Ser Val Ile Ala Gly Ala Val Ala Met Ala Val Val Ser 

                        | mature papA
                        | 1   2   3   4   5   6   7   8   9   10
 TTT GGT GTA AAT AAT GCT GCT CCA ACT ATT CCA CAG GGG CAG GGT AAA 
 Phe Gly Val Asn Asn Ala Ala Pro Thr Ile Pro Gln Gly Gln Gly Lys
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr L. Steidler(1) (2) and Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Steidler et al., J. Bacteriol. 175 (1993), 7639-7643 [PMID: 7902348]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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