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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pMxhFasR (LMBP 3345)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p3345.gb (View with Genome Compiler)
p3345.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human TNF receptor superfamily, member 6 cDNA (FAS)
Promoter: Mouse Mx promoter
Escherichia coli lac operon promoter
Phage SP6 promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells
Parental clone: pSP64MxpA; pCDM8hFasR
Further information: This plasmid was constructed by inserting the human Fas antigen/receptor cDNA (hFasR; XbaI fragment of pCDM8hFasR) between the XbaI sites of pSP64MxpA. The orientation of the inserted gene is sense relative to the Mx promoter.
The plasmid contains the mouse Mx promoter including 19 nucleotides downstream from the TATA-box, ending at position -1 which is the middle of the lost PvuII site.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727469.1.
Other name of the plasmid is pMxFas.
EMBL Accession number: LT727469.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 12/03/1999
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, HindIII and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by D. Vercammen(1)(2) and Prof. Dr W. Fiers(1)(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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