Last data update: 24 November 2020 04:18 CET
Plasmid name: pMxhFasR (LMBP 3345)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Cloned DNA:||Human TNF receptor superfamily, member 6 cDNA (FAS)|
|Promoter:||Mouse Mx promoter
Escherichia coli lac operon promoter
Phage SP6 promoter
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli
|Parental clone:||pSP64MxpA; pCDM8hFasR|
|Further information:||This plasmid was constructed by inserting the human Fas antigen/receptor cDNA (hFasR; XbaI fragment of pCDM8hFasR) between the XbaI sites of pSP64MxpA. The orientation of the inserted gene is sense relative to the Mx promoter.
The plasmid contains the mouse Mx promoter including 19 nucleotides downstream from the TATA-box, ending at position -1 which is the middle of the lost PvuII site.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727469.1.
Other name of the plasmid is pMxFas.
|EMBL Accession number:||LT727469.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||12/03/1999|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI, HindIII and XbaI.|
|History of deposit:||This plasmid was deposited by D. Vercammen(1)(2) and Prof. Dr W. Fiers(1)(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.