GREAT AT SMALL THINGS

0

LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMxh5HT2R (LMBP 3029)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p3029.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human serotonin 5-HT2 receptor (h5HT2R)
Promoter: Mouse Mx promoter
Escherichia coli lac operon promoter
Phage SP6 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells
Parental clone: pSP64MxpA; UCh5HT2R
Further information: The plasmid was constructed as follows : pSP64MxpA was digested with XbaI / BglI and with BamHI / BglI to isolate the Mx-promoter and the SV40 intron and the polyA-sequences respectively. The human serotonin 5HT2 receptor was isolated as a NheI/BamHI fragment from pUCh5HT2R. Ligation of the three fragments resulted in pMxh5HT2R. This plasmid allows functional expression of the human serotonin 5HT2 receptor in L929s(A). The Mx promoter is inducible with IFNα and IFNβ.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
Other name of the plasmid is pMx5HT2.
EMBL Accession number: -
Latest sequence update: 10/08/1994
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search