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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMxCAT (LMBP 3121)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pmxcat.gb (View with Genome Compiler)
pmxcat.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Chloramphenicol acetyltransferase gene (cat)
Promoter: Mouse Mx promoter
Escherichia coli lac operon promoter
Phage SP6 promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Mammalian cells
Parental clone: pSP64MxpA; pBLCAT5
Further information: This plasmid was constructed by inserting the Sau3AI fragment from pBLCAT5, containing the chloramphenicol acetyltransferase gene (cat), into the unique BamHI site of pSP64MxpA.
The mouse Mx promoter fragment includes 19 nucleotides downstream from the TATA-box (up to the lost PvuII site).
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727443.1.
EMBL Accession number: LT727443.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 07/11/1994
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoNI, HindIII, NcoI and ScaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr V. Vandevoorde(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Vandevoorde et al., J. Cell Biol. 137 (1997), 1627-1638 [PMID: 9199176]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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