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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pMcTrpMTNF (LMBP 2351)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2351.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor cDNA (Tnf, TNF); mature sequence
Promoter: Escherichia coli tryptophan operon promoter (trp)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)
Terminator: Phage fd terminator
Selection marker: Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMc58; pATTrpMTNF
Further information: This plasmid was constructed by inserting the PstI-XbaI fragment of pATTrpMTNF (containing mature mTnf and the
trp promoter) into the PstI and unique XbaI sites of pMc58 (partial digest with PstI). The unique SalI site of the polylinker of pMc58 has been deleted. The plasmid has resistance to CAM, and is in a Sup-strain sensitive to AMP.
The exact sequence between the PstI site and the lost PvuII site of the Trp promoter sequence is unknown.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
EMBL Accession number: M11731, view at EMBL, GenBank, DDBJ
Latest sequence update: 17/01/1992
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH1(λ)
Host reference: Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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