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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMcT4HTNFm50F (LMBP 2632)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2632.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human tumor necrosis factor cDNA (TNF); mutated mature sequence
Promoter: Phage T4 gene 32 promoter (T4g32)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T4 gene 32 (T4g32)
Terminator: Phage fd terminator
Escherichia coli tryptophan operon terminator (trp); synthetic sequence
Selection marker: Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMa-T4-hTNF-TA
Further information: The plasmid was constructed by site-specific mutagenesis of pMa-T4-hTNF-TA. As a result, codon 50 (GTG) of mature hTNF encoding valine (V) was replaced by TTC encoding phenylalanine (F).
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
The nucleotide sequence of the wt hTNF cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X01394.1.
EMBL Accession number: X01394.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 20/01/1993
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 SURE
Host reference: Greener, Strategies 3 (1990), 5-6
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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