LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMcSPA2 (LMBP 1884)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence:
analysis results


Cloned DNA: Staphylococcus aureus protein A cDNA (spa); mutated fragment
Promoter: -
binding site:
Terminator: Phage fd terminator
Selection marker: Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMaSPA1
Further information: This plasmid was constructed by site-specific mutagenesis of pMaSPA1, such that two BamHI sites were created.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
Other name of the plasmid is pMc58SPA-A.
EMBL Accession number: -
Latest sequence update: 06/05/1993
Sequence detail:
Nucleotide sequence of the A domain of the mutated S. aureus spa gene:

      ----- spaD ----> ---------------- spaA ----------------> ----- spaB ---->
       162         165 166     168 169 170     220         223 224     226 227 
       Gln Ala Pro Lys Ala Asp Pro Asn Phe     Gln Ala Pro Lys Ala Asp Pro Lys
                         ^     ^^                                BamHI ^^

Nucleotide sequence of the A domain of the wild type S. aureus spa gene:

      ----- spaD ----> ---------------- spaA ----------------> ----- spaB ---->
       162         165 166     168 169 170     220         223 224     226 227 
       Gln Ala Pro Lys Ala Asp Asn Asn Phe     Gln Ala Pro Lys Ala Asp Asn Lys

^: mutated nucleotide.
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr L. Steidler(1) (2) and Prof. Dr E. Remaut(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Steidler et al., Mol. Gen. Genet. 236 (1993), 187-192 [PMID: 8437563]
Related plasmid reference: Uhlén et al., J. Biol. Chem. 259 (1984), 1695-1702 [PMID: 6319407]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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