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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMcMTNFm73 (LMBP 2685)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2685.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor cDNA (Tnf, TNF); with deletion in the presequence
Promoter: -
Ribosome
binding site:
-
Terminator: Phage fd terminator
Selection marker: Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMaMTNF
Further information: This plasmid was constructed by site-specific mutagenesis of pMaMTNF; the nucleotide sequence of codon 73 of the presequence of mouse TNF has been modified (silent mutation) to create a unique NcoI restriction site.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
The nucleotide sequence of the wild type mouse TNF corresponds with the EMBL Nucleotide Sequence Database accession number M11731.1.
Other name of the plasmid is pMcMTNFmutNcoI
EMBL Accession number: M11731.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 26/01/1993
Sequence detail:
mouse TNF  wild type:

                    presequence           |  mature mTNF       
                73                      79| 1   2   3   4
5' CTC ATC AGT TCT ATG GCC CAG ACC CTC ACA CTC AGA TCA TCT ... 3'
   Leu Ile Ser Ser Met Ala Gln Thr Leu Thr Leu Arg Ser Ser

mouse TNF mutant:

                73                      79  1   2   3   4
5' CTC ATC AGT TCC ATG GCC CAG ACC CTC ACA CTC AGA TCA TCT ... 3'
   Leu Ile Ser Ser Met Ala Gln Thr Leu Thr Leu Arg Ser Ser
                NcoI
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr E. Decoster(1) (2) and Prof. Dr W. Fiers(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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