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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMcMTNFMLT (LMBP 2688)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2688.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor cDNA (Tnf); presequence
Mouse lymphotoxin A (Lta); mature sequence
Promoter: -
Ribosome
binding site:
-
Terminator: Phage fd terminator
Selection marker: Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMaMLTm3; pMcMTNFm73
Further information: This plasmid was constructed as follows : the SplI-HindIII fragment of pMaMLTm3 (containing mature mouse Lta) was ligated to the large NcoI-HindIII fragment of pMcMTNFm73 (containing the ori, cam and the mouse Tnf presequence) by insertion of a synthetic linker
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
EMBL Accession number: M11731, view at EMBL, GenBank, DDBJ
M16819, view at EMBL, GenBank, DDBJ
Latest sequence update: 19/05/1992
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
The plasmid was constructed by Dr Els Decoster.
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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