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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMcMLT (LMBP 2686)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2686.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse lymphotoxin A (Lta, LT); mature sequence
Promoter: -
Ribosome
binding site:
-
Terminator: Phage fd terminator
Selection marker: Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMc58; pM7A
Further information: The plasmid was constructed by inserting the EcoRI-BamHI fragment of pM7A (containing mature MLT) between the unique EcoRI and unique BamHI sites of pMc58
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
See also pM7A and pMc58
EMBL Accession number: BC099464, view at EMBL, GenBank, DDBJ
Latest sequence update: 23/01/1992
Sequence detail:
Sequence at the start of mature mLta:

           Sig seq | mature mLta
mLta wt     CAG.GGA|CTC.TCT.GGT.GTC.CGC.TTC.TCC.GCT.GCC.AGG
            Gln Gly|Leu Ser Gly Val Arg Phe Ser Ala Ala Arg

pMcMLT   GAATTC ATG.CTC.TCT.GGT.GTC.CGC.TTC.TCC.GCT.GCC.AGG
                Met Leu Ser Gly Val Arg Phe Ser Ala Ala Arg

          EcoRI                 SplI
pMaMLTm3 GAATTC.ATG.CTC.TCT.GGC.GTA.CGC.TTC.TCC.GCT.GCC.AGG
                              *   *
                Met Leu Ser Gly Val Arg Phe Ser Ala Ala Arg

* : mutated nucleotide
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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