Last data update: 28 November 2020 04:24 CET
Plasmid name: pMac254mSA (LMBP 2149)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Terminator:||Phage fd terminator|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli|
|Parental clone:||pMc254mSA; pMa254|
|Further information:||This phasmid was derived from pMc254mSA by replacing the BglI-AhaII fragment from the mutated ampicillin sensitive gene (AMPsm) by the corresponding fragment from the ampicillin resistant gene (AMPr) of pMa254, eliminating the internal amber mutation. This means that the plasmid can be used for ampicillin selection in a sup(-) host strain, in which the mutated β-lactamase is 11 amino acids longer than the natural form and provides resistance to ampicillin.
The f1 part of the plasmid contains the f1-origin, but also the sequence around the gene II promoter; the two trp terminators at the end of the chloramphenicol gene are not present.
Other name of the plasmid is pMac254SA.
|EMBL Accession number:||-|
|Latest sequence update:||01/08/1990|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by K. De Sutter(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + chloramphenicol (25 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.