LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMac254mSA (LMBP 2149)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence:
analysis results


Cloned DNA: -
Promoter: -
binding site:
Terminator: Phage fd terminator
Selection marker: Ampicillin (amp)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMc254mSA; pMa254
Further information: This phasmid was derived from pMc254mSA by replacing the BglI-AhaII fragment from the mutated ampicillin sensitive gene (AMPsm) by the corresponding fragment from the ampicillin resistant gene (AMPr) of pMa254, eliminating the internal amber mutation. This means that the plasmid can be used for ampicillin selection in a sup(-) host strain, in which the mutated β-lactamase is 11 amino acids longer than the natural form and provides resistance to ampicillin.
The f1 part of the plasmid contains the f1-origin, but also the sequence around the gene II promoter; the two trp terminators at the end of the chloramphenicol gene are not present.
Other name of the plasmid is pMac254SA.
EMBL Accession number: -
Latest sequence update: 01/08/1990
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by K. De Sutter(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + chloramphenicol (25 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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