Last data update: 28 November 2020 04:24 CET
Plasmid name: pMaT4HTNFd1-7 (LMBP 2647)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human tumor necrosis factor cDNA (TNF); fragment|
|Promoter:||Phage T4 gene 32 promoter (T4g32)|
|Ribosome binding site (RBS) of the phage T4 gene 32 (T4g32)|
|Terminator:||Phage fd terminator
Escherichia coli tryptophan operon terminator (trp); synthetic sequence
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli|
|Further information:||The plasmid was constructed by replacing the 34 nucleotide fragment resulting from a ClaI-partial AvaI digest by an oligo. In this way the first 7 AA of mature hTNF were eliminated, and a new initiation codon was created before AA 8. One AvaI site was lost as well, leaving one restriction site unique in the plasmid.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
Other name of the plasmid is pMaT4HTNFTd1-7.
|EMBL Accession number:||-|
|Latest sequence update:||07/01/1993|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Van Ostade et al., Protein Eng. 7 (1994), 5-22 [PMID: 8140094]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 SURE|
|Host reference:||Greener, Strategies 3 (1990), 5-6
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.