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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMaSVTHIL62 (LMBP 2332)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p2332.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Simian virus 40 (SV40) gene encoding large T-antigen
Promoter: Human interleukin 6 promoter (IL6)
Ribosome
binding site:
-
Terminator: Phage fd terminator
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMaSVT2; pGEMIL6gIL2f1
Further information: The plasmid was constructed by replacing the EcoRI-XhoI fragment from pMaSVT2 (containing the SV40-E promoter) by the EcoRI-XhoI fragment from pGEMIL6gIL2f1. Hence, the SV40 large T-antigen is placed under control of the human interleukin 6 promoter.
This plasmid differs from pMaSVTHIL61 in the length of the 5' flanking region of the hIL6 promoter.
pMaSVTHIL62 has 12 nucleotides more and 3 BamHI sites, instead of 2.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
Name of the phasmid mentioned in Vanhoenacker et al. (1994) is pMaHIL6SVT.
The nucleotide sequence of the genomic hIL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11. However, the C nucleotide at promoter position -174 (polymorphism mutant) was changed into a G nucleotide (wild type).
EMBL Accession number: AC073072.11, view at EMBL, GenBank, DDBJ
Latest sequence update: 30/04/2004
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Vanhoenacker et al., Eur. Cytok. Netw. 5 (1994), 283-291 [PMID: 7948766]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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