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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMaSVT (LMBP 2329)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pmasvt.gb (View with Genome Compiler)
pmasvt.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Simian virus 40 (SV40) gene encoding large T-antigen
Promoter: Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Phage fd terminator
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pMa58; pSV51
Further information: This phasmid was constructed by inserting the EcoRI-XbaI fragment from pSV51, containing the SV40 early promoter and the SV40 large T-antigen, into the EcoRI and XbaI sites of pMa58.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727495.1.
EMBL Accession number: LT727495.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 19/03/1991
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AvaII, BamHI/HindIII and HindII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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