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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMaMTNF (LMBP 2347)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pmamtnf.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse tumor necrosis factor cDNA (Tnf, TNF)
Promoter: -
Ribosome
binding site:
-
Terminator: Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMa58; pSV23SMTNF
Further information: This plasmid was constructed as follows: the SalI fragment of pSV23SMTNF (containing cDNA of the mouse TNF gene) was inserted clockwise into the unique SalI site of pMa58.
Restriction enzyme digestions revealed that approx. 60 nucleotides are missing from the 3' untranslated region of mouse TNF, including the 3'-terminal (supposedly restored) EcoRI site.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
The nucleotide sequence of the mouse TNF corresponds with the EMBL Nucleotide Sequence Database accession number M11731.1.
Other name of the plasmid is pMamTNF.
EMBL Accession number: M11731.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 26/01/1993
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr W. Fiers(1) (2). It was constructed by X. Van Ostade(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH1(λ)
Host reference: Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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