Last data update: 28 November 2020 04:24 CET
Plasmid name: pMaMTNF (LMBP 2347)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse tumor necrosis factor cDNA (Tnf, TNF)|
|Terminator:||Phage fd terminator|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli|
|Parental clone:||pMa58; pSV23SMTNF|
|Further information:||This plasmid was constructed as follows: the SalI fragment of pSV23SMTNF (containing cDNA of the mouse TNF gene) was inserted clockwise into the unique SalI site of pMa58.
Restriction enzyme digestions revealed that approx. 60 nucleotides are missing from the 3' untranslated region of mouse TNF, including the 3'-terminal (supposedly restored) EcoRI site.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
The nucleotide sequence of the mouse TNF corresponds with the EMBL Nucleotide Sequence Database accession number M11731.1.
Other name of the plasmid is pMamTNF.
|EMBL Accession number:||M11731.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||26/01/1993|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr W. Fiers(1) (2). It was constructed by X. Van Ostade(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH1(λ)|
|Host reference:||Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.