Last data update: 28 November 2020 04:24 CET
Plasmid name: pMaMIL4 (LMBP 2023)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Mouse interleukin 4 cDNA (IL4)|
|Terminator:||Phage fd terminator|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli|
|Parental clone:||pMa58; pSP65MIL4|
|Further information:||pMaMIL4 provides resistance to ampicillin, but is sensitive to chloramphenicol.
The phasmid contains the replication origin of the single-stranded DNA phage f1, so that it can be rapidly switched between the plasmid and the phage mode (ssDNA) of replication. The latter requires infection with a helper phage, e.g. M13KO7.
The phasmid was designed for site-specific mutagenesis where the mutated sequence can be physically linked with a selectable marker (cam). More than one mutagenesis round is possible via switching from ampicillin resistance to chloramphenicol resistance.
Since the length and the sequence of the 5' and 3' untranslated regions of the mIL4 gene are not exactly known, there is uncertainty as to the cutting frequency of the restriction enzymes indicated.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
pMaMIL4 contains the mouse interleukin 4 gene (mIL4) with flanking regions, a fragment of approx. 600 bp; this segment has not been sequenced (Lee et al. (1986)), but is biologically active.
|EMBL Accession number:||-|
|Latest sequence update:||02/06/1989|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by F. Hanssens(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Related plasmid reference:||Lee et al., Proc. Natl. Acad. Sci. U.S.A. 83 (1986), 2061-2065 [PMID: 3083412]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.