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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMaMIL4 (LMBP 2023)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pmamil4.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse interleukin 4 cDNA (IL4)
Promoter: -
Ribosome
binding site:
-
Terminator: Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMa58; pSP65MIL4
Further information: pMaMIL4 provides resistance to ampicillin, but is sensitive to chloramphenicol.
The phasmid contains the replication origin of the single-stranded DNA phage f1, so that it can be rapidly switched between the plasmid and the phage mode (ssDNA) of replication. The latter requires infection with a helper phage, e.g. M13KO7.
The phasmid was designed for site-specific mutagenesis where the mutated sequence can be physically linked with a selectable marker (cam). More than one mutagenesis round is possible via switching from ampicillin resistance to chloramphenicol resistance.
Since the length and the sequence of the 5' and 3' untranslated regions of the mIL4 gene are not exactly known, there is uncertainty as to the cutting frequency of the restriction enzymes indicated.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
pMaMIL4 contains the mouse interleukin 4 gene (mIL4) with flanking regions, a fragment of approx. 600 bp; this segment has not been sequenced (Lee et al. (1986)), but is biologically active.
EMBL Accession number: -
Latest sequence update: 02/06/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by F. Hanssens(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Lee et al., Proc. Natl. Acad. Sci. U.S.A. 83 (1986), 2061-2065 [PMID: 3083412]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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