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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMa58 (LMBP 1461)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pma58.gb (View with Genome Compiler)
pma58.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pMac5-8
Further information: The plasmid was designed for site-specific mutagenesis where the mutated sequence can be physically linked with a selectable marker (chloramphenicol).
The plasmid provides resistance to ampicillin, but is sensitive to chloramphenicol.
More than one mutagenesis round is possible via switching from ampicillin resistance to chloramphenicol resistance.
pMa58 contains the replication origin of the single-stranded DNA phage f1 (clockwise orientated), so that it can be rapidly switched between the plasmid and the phage mode (ssDNA) of replication. The latter requires infection with a helper phage, e.g. M13KO7.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
Name mentioned in Stanssens et al. (1989) is pMa5-8.
EMBL Accession number: -
Latest sequence update: 19/05/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglI/NgoMIV, EcoRI, HindII, PstI and ScaI. The plasmid seems to be approx. 200 bp larger as compared to the compiled nucleotide sequence.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. Stanssens(1).
(1) Corvas International N.V., Ghent, Belgium
Plasmid reference: Stanssens et al., Nucleic Acids Res. 17 (1989), 4441-4454 [PMID: 2501754]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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