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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMVVPGalER (LMBP 3301)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd)
Human estrogen receptor (ER) hormone binding domain
Herpes simplex viral protein VP16 transcriptional activation domain (VPad, VP16ad)
Promoter: Moloney mouse sarcoma virus long terminal repeat (MoMSV-LTR)
Herpes simplex virus (HSV) thymidine kinase promoter (TK)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Polyoma virus origin
Host range: Escherichia coli
Parental clone: pMV7; pSP64VPGalER
Further information: The plasmid was presumably constructed by inserting the VP-Gal-ER fusion gene as HindIII fragment into the unique HindIII site of pMV-7.
The VP-Gal-ER fusion gene encodes the DNA-binding domain of the Saccharomyces cerevisiae Gal4 protein fused to the hormone binding domain of the human estrogen receptor (ER). This Gal-ER fusion is converted to a transcription factor by fusion to the strong activation domain of the herpes simplex protein VP16. The fusion of VP16 at the N-terminus of Gal-ER (VP-Gal-ER) seems to give less good promoter induction as compared to the fusion of VP16 at the C-terminus (Gal-ER-VP).
EMBL Accession number: -
Latest sequence update: 04/02/1997
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr S. Braselmann(1).
(1) Onyx Pharma., Richmond, USA
Plasmid reference: -
Related plasmid reference: Braselmann et al., Proc. Natl. Acad. Sci. U.S.A. 90 (1993), 1657-1661 [PMID: 8446579]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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