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LMBP plasmid details

Last data update: 24 November 2020 04:18 CET

Plasmid name: pMSG-MAC1 (LMBP 3093)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p3093.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse cadherin 1 cDNA (Cdh1, E-cad, CDH1); antisense
Promoter: Mouse mammary tumor virus long terminal repeat (MMTV-LTR)
Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Xanthine-guanine phosphoribosyltransferase (XGPRT)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli; preferably recombination-deficient strains
Mammalian cells; SV40 permissive cells
Parental clone: pMSG; pBATEM2
Further information: The plasmid was constructed by ligating a 714 bp XhoI/XmaI fragment of pBATEM2 in the XhoI/XmaI-opened pMSG. This fragment contains nucleotides 1604 to 2318 of mCdh1, which encodes antisense RNA.
EMBL Accession number: -
Latest sequence update: 12/10/2010
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by K. Staes(1) (2), Prof. Dr K. Vleminckx(1) (2) and Prof. Dr F. Van Roy(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Vleminckx et al., Cell 66 (1991), 107-119 [PMID: 2070412]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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