Last data update: 28 November 2020 04:24 CET
Plasmid name: pMSCV-VSV-hPIDD1-FLAG (LMBP 7791)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Depositor's sequence:||not available|
|Cloned DNA:||Human p53-induced death domain protein 1 cDNA (PIDD1, LRDD, PIDD)
Vesicular stomatitis virus glycoprotein (VSV-G) epitope; N-terminal
FLAG epitope tag; C-terminal
|Promoter:||Mouse stem cell PCMV virus long terminal repeat (5' MSCV-PCMV LTR)
Mouse phosphoglycerate kinase 1 promoter (PGK1)
Escherichia coli lac operon promoter
|Terminator:||Mouse stem cell PCMV virus long terminal repeat (3' MSCV-PCMV LTR)|
|Selection marker:||Puromycin (puro)
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; preferably recombination-deficient strains
|Further information:||The plasmid was constructed by PCR amplifying the 5' region of human PPID1 from IMAGE clone 3908102 and the 3' region from IMAGE clone 4852968. A 5' VSV-tag and a 3' FLAG epitope were added during PCR amplification. The human PPID1 coding sequence was fused, and cloned into the pMSCVpuro vector.
The plasmid contains the mouse stem cell PCMV (PCC4-cell-passaged myeloproliferative sarcoma virus) virus long terminal repeat. The LTR drives high-level constitutive expression of human PIDD1 in stem cells and other mammalian cell lines.
The plasmid contains also the extended retroviral packaging signal, Ψ+, which promotes high-titer production of replication-incompetent infectious viral particles after introduction into a packaging cell line.
Other name of the plasmid is pMSCV-PIDDisof1-Flag.
|EMBL Accession number:||-|
|Latest sequence update:||28/06/2018|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr P Schneider(1).
(1) Department of Biochemistry, University of Lausanne, Epalinges, Switzerland
|Plasmid reference:||Tinel et al., Science 304 (2004), 843-846 [PMID: 15073321]
|Related plasmid reference:||Cuenin et al., Oncogene 27 (2008), 387-396 [PMID: 17637755]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.