LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMSCV-VSV-hPIDD1-FLAG (LMBP 7791)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
analysis results


Cloned DNA: Human p53-induced death domain protein 1 cDNA (PIDD1, LRDD, PIDD)
Vesicular stomatitis virus glycoprotein (VSV-G) epitope; N-terminal
FLAG epitope tag; C-terminal
Promoter: Mouse stem cell PCMV virus long terminal repeat (5' MSCV-PCMV LTR)
Mouse phosphoglycerate kinase 1 promoter (PGK1)
Escherichia coli lac operon promoter
binding site:
Terminator: Mouse stem cell PCMV virus long terminal repeat (3' MSCV-PCMV LTR)
Selection marker: Puromycin (puro)
Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient strains
Mammalian cells
Parental clone: pMSCVpuro
Further information: The plasmid was constructed by PCR amplifying the 5' region of human PPID1 from IMAGE clone 3908102 and the 3' region from IMAGE clone 4852968. A 5' VSV-tag and a 3' FLAG epitope were added during PCR amplification. The human PPID1 coding sequence was fused, and cloned into the pMSCVpuro vector.
The plasmid contains the mouse stem cell PCMV (PCC4-cell-passaged myeloproliferative sarcoma virus) virus long terminal repeat. The LTR drives high-level constitutive expression of human PIDD1 in stem cells and other mammalian cell lines.
The plasmid contains also the extended retroviral packaging signal, Ψ+, which promotes high-titer production of replication-incompetent infectious viral particles after introduction into a packaging cell line.
Other name of the plasmid is pMSCV-PIDDisof1-Flag.
EMBL Accession number: -
Latest sequence update: 28/06/2018
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr P Schneider(1).
(1) Department of Biochemistry, University of Lausanne, Epalinges, Switzerland
Plasmid reference: Tinel et al., Science 304 (2004), 843-846 [PMID: 15073321]
Related plasmid reference: Cuenin et al., Oncogene 27 (2008), 387-396 [PMID: 17637755]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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