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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMSCV-F-BirA (LMBP 5689)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5689.gb (View with Genome Compiler)
p5689.txt
p5689.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli biotin protein ligase cDNA (birA)
FLAG epitope tag; N-terminal
Promoter: Mouse stem cell PCMV virus long terminal repeat (5' MSCV-PCMV LTR)
Mouse phosphoglycerate kinase 1 promoter (PGK1)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Mouse stem cell PCMV virus long terminal repeat (3' MSCV-PCMV LTR)
Selection marker: Ampicillin (amp)
Puromycin (puro)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; preferably recombination-deficient strains
Mammalian cells
Parental clone: pMSCV-FLAG-puromycin
Further information: The plasmid contains the mouse stem cell PCMV (PCC4-cell-passaged myeloproliferative sarcoma virus) virus long terminal repeat. The LTR drives high-level constitutive expression of BirA in stem cells and other mammalian cell lines.
The plasmid contains also the extended retroviral packaging signal, Ψ+, which promotes high-titer production of replication-incompetent infectious viral particles after introduction into a packaging cell line.
The plasmid is used in cotransfection with clones carrying the bio-tag for purification of biotinylated proteins.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726944.1.
Other names of the plasmid are MSCV-BirA and MSCVp-F-BirA.
EMBL Accession number: LT726944.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/11/2007
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AatII/KpnI, Alw44I/XbaI, Bst98I, NcoI, PvuII/SpeI, SphI, SspI and Tth111I.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. Baens(1).
(1) Department of Human Genetics, KULeuven, Belgium
Plasmid reference: Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209]
Related plasmid reference: Muhammad et al., Front Immunol 9 (2018), 32 [PMID: 29416540] [DOI: 10.3389/fimmu.2018.00032]
Alrefai et al., Nat Commun 7 (2016), 11724 [PMID: 27222343] [DOI: 10.1038/ncomms11724]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1 in E. coli; L2 in mammalian cells
Cultivation remark: Take a low-copy-plasmid yield into account while choosing a plasmid DNA isolation procedure.
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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