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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMS3 (LMBP 9304)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Yersinia enterocolitica anti-host protein E gene (yopE)
Yersinia enterocolitica specific yopE chaperone gene (sycE, yerA)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Chloramphenicol (cam)
Replicon: Broad-host-range plasmid RK2 origin of transfer (oriT)
Escherichia coli plasmid p15A origin
Host range: Escherichia coli
Gram-negative bacterial strains
Parental clone: pTM100; pYVe439-80
Further information: The plasmid was constructed by cloning a 1745 bp XhoI fragment from pYVe439-80, containing the Y. enterocolitica yopE and sycE coding sequences, into the pTM100 vector.
Other names of the plasmid are pTM100:yopE+-sycE+ and pACYC184 oriTRK2 + yopE, sycE.
EMBL Accession number: -
Latest sequence update: 03/07/2014
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr M.-P. Sory(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Microbial Pathogenesis Unit, Institute of Cellular and Molecular Pathology, Catholic University of Louvain, Brussels, Belgium
Plasmid reference: Sory et al., Infect. Immun. 60 (1992), 3830-3836 [PMID: 1500192]
Related plasmid reference: Michiels et al., J. Bacteriol. 173 (1991), 1677-1685 [PMID: 1999387]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 S17-1
Host reference: -
Related host reference: Simon et al., Biotechnology 1 (1983), 784-791
Cultivation medium: LB-Lennox + chloramphenicol (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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