Last data update: 28 November 2020 04:24 CET
Plasmid name: pMS3 (LMBP 9304)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Depositor's sequence:||not available|
|Cloned DNA:||Yersinia enterocolitica anti-host protein E gene (yopE)
Yersinia enterocolitica specific yopE chaperone gene (sycE, yerA)
|Selection marker:||Chloramphenicol (cam)|
|Replicon:||Broad-host-range plasmid RK2 origin of transfer (oriT)
Escherichia coli plasmid p15A origin
|Host range:||Escherichia coli
Gram-negative bacterial strains
|Parental clone:||pTM100; pYVe439-80|
|Further information:||The plasmid was constructed by cloning a 1745 bp XhoI fragment from pYVe439-80, containing the Y. enterocolitica yopE and sycE coding sequences, into the pTM100 vector.
Other names of the plasmid are pTM100:yopE+-sycE+ and pACYC184 oriTRK2 + yopE, sycE.
|EMBL Accession number:||-|
|Latest sequence update:||03/07/2014|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr M.-P. Sory(2).
(1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium
(2) Microbial Pathogenesis Unit, Institute of Cellular and Molecular Pathology, Catholic University of Louvain, Brussels, Belgium
|Plasmid reference:||Sory et al., Infect. Immun. 60 (1992), 3830-3836 [PMID: 1500192]
|Related plasmid reference:||Michiels et al., J. Bacteriol. 173 (1991), 1677-1685 [PMID: 1999387]
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 S17-1|
|Related host reference:||Simon et al., Biotechnology 1 (1983), 784-791
|Cultivation medium:||LB-Lennox + chloramphenicol (10 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.