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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMS27 (LMBP 5349)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5349.gb (View with Genome Compiler)
p5349.txt
p5349.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Phage MS2 genome; cDNA
Escherichia coli insertion sequence IS1 insB
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322; MS2
Further information: This plasmid contains a nearly full copy (the first 14 nucleotides are missing) of the phage MS2 genome, obtained by cDNA synthesis, and cloned via dT-tailing in the dA-tailed PstI site of pBR322. The exact length of the dA/dT-tails is not known.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727009.1.
Name mentioned in Devos et al. (1979) is pMS2-7.
EMBL Accession number: LT727009.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 24/03/1992
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AvrII/SphI, BamHI, BglI/EcoRV, NheI, NheI/PstI, PstI, ScaI and SspI.

The sequence of the MS2 cDNA has not been checked (sequenced) on possible mistakes, but the restriction pattern fits the original RNA sequence.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr R. Devos(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Devos et al., J. Mol. Biol. 128 (1979), 595-619 [PMID: 374745]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: LMBP 00266

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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