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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMOL322 (LMBP 8043)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Neomycin (neo; kanamycin (kan))
Tetracycline (tet)
Pseudomonas ARGI; auxotrophic
Pseudomonas ARGF; auxotrophic
Replicon: -
Host range: Escherichia coli
Parental clone: pULB113
Further information: The plasmid was constructed in vivo based on natural plasmids.
The plasmid belongs to the IncP/P-1 incompatibility family.
pMOL322 contains prophage Mu3A (mini-Mu).
EMBL Accession number: -
Latest sequence update: 18/03/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by M. Mergeay(1) and A. Provoost(1).
(1) SCK.CEN, Mol, Belgium
Plasmid reference: -
Related plasmid reference: Faelen et al., Cold Spring Harb. Symp. Quant. Biol. 43 (1979), 1169-1177 [PMID: 158471] [DOI: 10.1101/SQB.1979.043.01.132]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MXR
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: SCK CM0299

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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