GREAT AT SMALL THINGS

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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMOL112 (LMBP 8036)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Streptomycin (Sm)
Replicon: -
Host range: Escherichia coli
Yeast; Saccharomyces, Kluyveromyces, Pichia, Candida, Yarrovia, etc.
Parental clone: pGT30
Further information: The plasmid was constructed by cloning a 800 bp BamHI fragment containing ribosomal DNA encoding 25S RNA of A. thaliana, into the BamHI opened pGT30 vector.
EMBL Accession number: -
Latest sequence update: 18/03/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by M. Mergeay(1) and A. Provoost(1).
(1) SCK.CEN, Mol, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB HB101
Host reference: Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
Related host reference: Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: SCK CM0284

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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