Last data update: 28 November 2020 04:24 CET
Plasmid name: pMMB24 (LMBP 1099)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Aminoglycoside phosphotransferase structural gene (aph); strA and strB genes (conferring streptomycin resistance) without promoter
Broad-host-range plasmid RSF1010 plasmid mobilization genes A, B and C (mobA, mobB, mobC)
Broad-host-range plasmid RSF1010 plasmid replication genes A, B and C (repA, repB, repC)
Broad-host-range plasmid RSF1010 repressor F gene (rpsF)
|Promoter:||Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)
Escherichia coli lac repressor promoter; mutant (lacI(q))
|Selection marker:||Ampicillin (amp)|
|Replicon:||Broad-host-range plasmid RSF1010 vegetative replication origin; IncQ
Broad-host-range plasmid RSF1010 origin of transfer (oriT)
|Host range:||Escherichia coli
Gram-negative bacterial strains
|Parental clone:||pMMB15; placIq|
|Further information:||The plasmid was constructed by isolating a 1.1 kb EcoRI fragment from placIq, carrying the lacIq gene and its promoter. The restriction ends were filled in using T4 DNA polymerase and EcoRI linkers were ligated to the resulting blunt ends. The lacIq fragment was subsequently inserted into the EcoRI-opened pMMB15 vector to give a self-repressible vector.
The plasmid is a broad host range expression vector controlled by the tac promoter.
|EMBL Accession number:||-|
|Latest sequence update:||10/03/2014|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr M. Bagdasariant(1).
(1) Department for Microbiology, Michigan State University, East Lansing, Michigan, USA
|Plasmid reference:||Bagdasarian et al., Gene 26 (1983), 273-282 [PMID: 6323265]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 SK1592|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.