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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMMB22 (LMBP 1098)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p1098.gb (View with Genome Compiler)
Sequence
analysis results
Genecorner:

-

Cloned DNA: Aminoglycoside phosphotransferase structural gene (aph); strA and strB genes conferring streptomycin resistance
Broad-host-range plasmid RSF1010 plasmid mobilization genes A, B and C (mobA, mobB, mobC)
Broad-host-range plasmid RSF1010 plasmid replication genes A, B and C (repA, repB, repC)
Broad-host-range plasmid RSF1010 repressor F gene (rpsF)
Promoter: Escherichia coli hybrid tryptophan/lacUV5 promoter (tac)
Escherichia coli lac repressor promoter; mutant (lacI(q))
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Streptomycin (Sm)
Replicon: Broad-host-range plasmid RSF1010 vegetative replication origin; IncQ
Broad-host-range plasmid RSF1010 origin of transfer (oriT)
Host range: Escherichia coli
Gram-negative bacterial strains
Parental clone: pMMB6; placIq
Further information: The plasmid was constructed by isolating a 1.1 kb EcoRI fragment from placIq, carrying the lacIq gene and its promoter. The restriction ends were filled in using T4 DNA polymerase and HindIII linkers were ligated to the resulting blunt ends. The lacIq fragment was subsequently inserted into the HindIII-opened pMMB6 vector to give a self-repressible vector.
pMMB22 is a broad host range expression vector controlled by the tac promoter.
It can be used in all gram-negative bacterial species that support replication of the natural plasmid RSF1010.
EMBL Accession number: -
Latest sequence update: 10/03/2014
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, EcoRV, HindIII and NcoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr M. Bagdasariant(1).
(1) Department for Microbiology, Michigan State University, East Lansing, Michigan, USA
Plasmid reference: Bagdasarian et al., Gene 26 (1983), 273-282 [PMID: 6323265]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 WM837
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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