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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMG11 (LMBP 8462)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p8462.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli lac Z gene (lacZ); with modified 5' end
Bacillus subtilis amylase gene (amyE); fragments
Promoter: Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Bacillus sp.; integrative
Parental clone: pMG6
Further information: The plasmid was constructed by PCR amplifying a 3.1 kb Acc65I/XbaI fragment containing the promoterless lacZ gene, and ligating it into the Acc65I/XbaI-opened pMG6 vector.
This plasmid is a modular E. coli–Bacillus shuttle vector, meant for the determination of promoter strength in B. subtilis via chromosomal integration of the lacZ gene and measuring β-galactosidase activity.
Furthermore, the modular architecture of the pMG plasmid family enables an easy exchange of all features like antibiotic resistance genes (EcoRI/SalI), promoters (EcoRI/PstI), 5′ UTR (PstI/HindIII) and tags (HindIII/Acc65I) leading to a significant increase of applicabilities.
The nucleotide sequence of the B. subtilis fragments correspond with the EMBL Nucleotide Sequence Database accession number AP012496.1.
EMBL Accession number: AP012496.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 06/06/2013
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI/SalI, HaeII, HincII and NotI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr. M. Gimpel(1) and Prof. Dr S. Brantl(1).
(1) AG Bakteriengenetik, FSU Jena, Jena, Germany
Plasmid reference: Gimpel et al., J. Microbiol. Methods 91 (2012), 312-317 [PMID: 22982324] [DOI: 10.1016/j.mimet.2012.09.003]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin.
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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