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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMET7hIL-2Ralpha (LMBP 5280)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5280.gb (View with Genome Compiler)
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 2 receptor subunit alpha cDNA (IL2RA, IDDM10, IL2R, CD25, GeneID 3559)
Promoter: Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα)
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pMET7
Further information: The plasmid contains the human interleukin 2 receptor α (hIL2Rα) coding sequence inserted downstream from the Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα) and from the phage T7 gene 10 promoter (T7g10).
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727001.1.
The nucleotide sequence of hIL2Rα was obtained from GenBank (Accession number X01057.1).
EMBL Accession number: X01057.1, view at EMBL, GenBank, DDBJ
LT727001.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 25/04/2006
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: KpnI, PstI and SacI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by E. Pattyn(1).
(1) Department of Biochemistry, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Takebe et al., Mol. Cell. Biol. 8 (1988), 466-472 [PMID: 2827008]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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