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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMET7-mLR (LMBP 7840)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p7840.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse leptin receptor cDNA (Lepr, LR, Obr)
Promoter: Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα)
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pMET7
Further information: The nucleotide sequence of the mouse Lepr cds and its 3' untranslated region corresponds with the Genbank accession number NM_001122899.1.
The region downstream of the T7 promoter and the region upstream of the SV40 polyA region was sequenced by LMBP.
Other name of the plasmid is pMET7 mLR short.
EMBL Accession number: NM_001122899.1, view at GenBank
Latest sequence update: 10/09/2012
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, KpnI, NotI/SalI and ScaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr R. Devos(1).
(1) Roche Research Gent, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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