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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMET7 (LMBP 5237)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα)
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pME18
Further information: Other name of the plasmid is pMet7 empty.
EMBL Accession number: -
Latest sequence update: 22/06/2006
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr J. Tavernier(1)(2).
(1) Center for Medical Biotechnology, VIB, Ghent, Belgium
(2) Department of Biochemistry, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Takebe et al., Mol. Cell. Biol. 8 (1988), 466-472 [PMID: 2827008]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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