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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMC1403 (LMBP 474)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: pmc1403.gb (View with Genome Compiler)
pmc1403.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli lac Z gene (lacZ); not functional
Escherichia coli lac Y gene (lacY)
Escherichia coli lac A gene (lacA)
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use lacZ(-) strains
Parental clone: pBR322
Further information: The plasmid can be used for cloning promoters with translation-initiation sequences and analyse their expression level by means of synthesis of an active β-galactosidase fusion protein.
The lacZ gene is not complete (the first 8 amino acids are missing).
The EcoRI site in the lacZ gene was removed.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727182.1.
Other name of the plasmid is pMC936.
EMBL Accession number: LT727182.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 17/03/1992
Sequence detail:
Nucleotide sequence in front of the lacZ gene:

                            9
5' GA.ATT.CCC.GGG.GAT.CCC.GTC.GTT 3'
   EcoRI  SmaI  BamHI      * 


*: Ninth codon of the lacZ gene.
Punctuation indicates reading frame.
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindII and SalI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M.J. Casadaban(1).
(1) University of Chicago, USA
Plasmid reference: Casadaban et al., J. Bacteriol. 143 (1980), 971-980 [PMID: 6162838]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 M182
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: NCCB 3088
PC-V 3088

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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