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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pMB7+pyrG (LMBP 5865)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Salmonella enterica serovar Typhimurium ATP phosphoribosyltransferase gene (hisG)
Trichoderma reesei uracil 3 gene (URA3)
Promoter: Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Trichoderma reesei URA3; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Trichoderma reesei; ura3(-), integrative
Parental clone: pMB-7; pFG1
Further information: The plasmid contains the Trichoderma reesei PyrG (pyr4, URA3) between repeating sequences of the Salmonella typhimurium HisG gene. The plasmid was created by removing the Candida URA3 gene from pMB-7 via restriction with NheI and BclI (T4 blunted). The URA3 gene was replaced by the Trichoderma reesei URA3 gene from pFG1 after restriction with SalI/T4.
EMBL Accession number: -
Latest sequence update: 17/03/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr N. Callewaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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