Last data update: 28 November 2020 04:24 CET
Plasmid name: pM6P-FLAG-mNEMO-R309A-R312A-E313A (LMBP 9750)
|Price category:||Cat. 2 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Depositor's sequence:||not available|
|Cloned DNA:||Mouse inhibitor of kappaB kinase gamma cDNA (Ikbkg, NEMO); mutated sequence
FLAG epitope tag; N-terminal
|Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Further information:||This retroviral expression plasmid was constructed by cloning the FLAG-tagged mouse IKBKG mutant into the pM6P vector.
The mouse Ikbkg coding sequence contains R309A, R312A and E313A mutations.
Information on this plasmid is very limited. Full sequence analysis is possible, but at an additional cost.
Other name of the plasmid is FLAG-NEMO F305 pM6P.
|EMBL Accession number:||-|
|Latest sequence update:||11/01/2017|
|Authenticity test:||This plasmid has been fully sequenced. The NGS sequence data still need to be implemented but were unfortunately incomplete.|
|History of deposit:||This plasmid was deposited by Prof. Dr I. Dikic(1).
(1) Institute of Biochemistry II, Goethe University School of Medicine, Frankfurt, Germany
|Restricted distribution:||- BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5α|
|Host reference:||Focus 8 (1986), 9
|Related host reference:||Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.