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LMBP plasmid details

Last data update: 28 November 2020 04:24 CET

Plasmid name: pM2-Apex2 (LMBP 10253)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: -
Sequence
analysis results
Genecorner:

-

Cloned DNA: Influenza A virus matrix protein 2 cDNA (M2)
Glycine max cytosolic ascorbate peroxidase 1 cDNA (APX1, GeneID 553156); improved monomeric enhanced mutant (APEX2)
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Simian virus 40 early promoter (SV40 early)
Escherichia coli β-lactamase promoter (amp)
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp)
Terminator: Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Neomycin (neo; G418; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pPTB
Further information: The plasmid was constructed by cloning the Influenza M2 and soybean APEX2 coding sequences into the pPTB vector.
APEX2, the second-generation monomeric enhanced APX mutant, contains an additional mutation (A134P) compared to APEX (K41D, W41F, E112K) and is used as a reporter to generate contrast for electron microscopy and also for proteomic tagging in living cells.
Other name of the plasmid is pM2Apex2.
EMBL Accession number: -
Latest sequence update: 17/08/2018
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr X. Saelens(1)(2)(3). It was constructed by L. Vande Ginste(1)(2)(3).
(1) Department of Medical Protein Research, VIB, Ghent, Belgium
(2) Inflammation Research Center, VIB, Ghent, Belgium
(3) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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