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LMBP plasmid details

Last data update: 25 September 2020 04:24 CEST

Plasmid name: pLoxGentrcmelA (LMBP 9150)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p9150.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Rhizobium etli CFN42 tyrosinase gene (melA)
Escherichia coli lac repressor gene (lacI)
Promoter: Escherichia coli hybrid tryptophan/lacUV5 promoter (trc)
Escherichia coli lac repressor promoter; mutant (lacI(q))
Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: Escherichia coli rrnB operon T1 terminator
Escherichia coli rrnB operon T2 terminator
Selection marker: Ampicillin (amp)
Gentamicin (Gm)
Replicon: Escherichia coli plasmid pMB1 origin
Bacteriophage M13 origin
Host range: Escherichia coli
Parental clone: pLoxGentrc
Further information: The plasmid was constructed by cloning the R. etli melA cds from pTrcmelA as a NcoI/SmaI fragment into the NcoI/SmaI opened pLoxGentrc vector.
pLoxGentrcmelA is meant for chromosomal gene integration and expression of the R. etli melA gene in E. coli, creating a genetically stable E. coli melanin production strain.
The nucleotide sequence of the pLoxGentrc vector corresponds with the EMBL Nucleotide Sequence Database accession number JF934954.1.
EMBL Accession number: JF934954.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 05/05/2014
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglI, NcoI/SmaI, PvuI and XhoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr Guillermo Gosset Lagarda(1). It was constructed by A. Sabido(1).
(1) Departamento de Ingenieria Celular y Biocatalisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Mexico
Plasmid reference: Sabido et al., Plasmid 69 (2013), 16-23 [PMID: 22884755] [DOI: 10.1016/j.plasmid.2012.04.005]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + gentamicin (10 μg/ml)*
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on gentamicin; subsequent cultivation of a single colony in liquid medium with ampicillin.
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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