Last data update: 26 September 2020 04:22 CEST
Plasmid name: pLacDN10HIFNG (LMBP 1575)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||Human interferon γ cDNA (IFNG)
Escherichia coli lac Z gene (lacZ); starting at the 9th codon
Escherichia coli lac Y gene (lacY)
Escherichia coli lac A gene (lacA)
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Further information:||pLacDN10HIFNG is derived from pLacDN1HIFNG by ligation of the filled in (with T4 DNA polymerase) unique BamHI site, so that the fusion protein human interferon γ/β-galactosidase is no longer in phase.
The natural EcoRI site near the end of lacZ was removed by mutation.
hIFNγ is a mutated variant, containing a unique SalI site and an extra HhaI site at position 390 of the coding region.
Other name of the plasmid is pDN10G.
|EMBL Accession number:||-|
|Latest sequence update:||09/02/1989|
Nucleotide sequence around the filled-in BamHI site: Arg Gly Arg Pro Ser Arg Ile Pro Gly Asp Arg Ser Val Val 5' CGA.GGT.CGA.CCC.TCT.AGA.ATT.CCC.GGG.GAT.CGA.TCC.C GTC.GTT 3' SalI XbaI EcoRI SmaI * XmaI *: 9th amino acid of the lacZ gene; phase shift. Punctuation indicates reading frame.
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by E. Sablon(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||PhD thesis Erwin Sablon (1990)
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061[pcI857]|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.