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LMBP plasmid details

Last data update: 23 September 2020 04:27 CEST

Plasmid name: pLT32hC14p10wt (LMBP 5338)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p5338.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human apoptosis-related cysteine peptidase 14 cDNA (CASP14, caspase 14, caspase-14, CASP-14); p10 subunit
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage T7 gene 10 terminator (T7g10)
Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pLT32
Further information: The plasmid was constructed by cloning the p10 subunit of human CASP14 into the pLT32 vector.
This low copy number plasmid can be used for IPTG-inducible expression of the p10 subunit of human CASP14 after transformation into an E. coli strain containing a compatible piCA plasmid.
Expression of the p10 subunit of human CASP14 can be offered by the Protein Service Facility (PSF), Inflammation Research Center, VIB-Ghent University, Belgium. Please contact BCCM/GeneCorner for your protein expression request or refer to BCCM/GeneCorner when contacting PSF directly.
The nucleotide sequence of the p10 subunit of human CASP14 corresponds with the EMBL Nucleotide Sequence Database accession number EU502748.1.
EMBL Accession number: EU502748.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 27/10/2006
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. De Groote(1) (2) and Prof. Dr W. Declercq(1) (2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Hoste et al., J. Invest. Dermatol. 131 (2011), 2233-2241 [PMID: 21654840]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061(λ)
Host reference: Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
Related host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -
Related website: http://www.irc.ugent.be/index.php?id=proteinservicehome

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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