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LMBP plasmid details

Last data update: 25 September 2020 04:24 CEST

Plasmid name: pLT32H-CNF1 (LMBP 4976)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli cytotoxic necrotizing factor type 1 cDNA (CNF1)
Histidine tag (His-tag); C-terminal
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage T7 gene 10 terminator (T7g10)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pLT32H; pHLK102
Further information: The plasmid was created by PCR amplifying the CNF1 fragment and ligating it into the pHLK102 vector. The cds was subsequently cloned as an XmaI fragment into the XmaI opened pLT32H vector.
This low copy number plasmid can be used for IPTG-inducible expression of human CNF1 after transformation into an E. coli strain containing a compatible piCA plasmid.
Expression of CFN1 with this vector can be offered by the Protein Service Facility (PSF), Inflammation Research Center, VIB-Ghent University, Belgium. Please contact BCCM/GeneCorner for your protein expression request or refer to BCCM/GeneCorner when contacting PSF directly.
EMBL Accession number: -
Latest sequence update: 30/03/2009
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L2
Other culture collection numbers: -
Related website: http://www.irc.ugent.be/index.php?id=proteinservicehomehttp://www.irc.ugent.be/index.php?id=proteinservicehome

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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