Last data update: 04 December 2020 04:17 CET
Plasmid name: pLT32H-CNF1 (LMBP 4976)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Depositor's sequence:||not available|
|Cloned DNA:||Escherichia coli cytotoxic necrotizing factor type 1 cDNA (CNF1)
Histidine tag (His-tag); C-terminal
|Promoter:||Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Phage λ major leftward promoter (λ PL)
|Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)|
|Terminator:||Phage T7 gene 10 terminator (T7g10)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Parental clone:||pLT32H; pHLK102|
|Further information:||The plasmid was created by PCR amplifying the CNF1 fragment and ligating it into the pHLK102 vector. The cds was subsequently cloned as an XmaI fragment into the XmaI opened pLT32H vector.
This low copy number plasmid can be used for IPTG-inducible expression of human CNF1 after transformation into an E. coli strain containing a compatible piCA plasmid.
Expression of CFN1 with this vector can be offered by the Protein Service Facility (PSF), Inflammation Research Center, VIB-Ghent University, Belgium. Please contact BCCM/GeneCorner for your protein expression request or refer to BCCM/GeneCorner when contacting PSF directly.
|EMBL Accession number:||-|
|Latest sequence update:||30/03/2009|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr R. Beyaert(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.