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LMBP plasmid details

Last data update: 26 September 2020 04:22 CEST

Plasmid name: pLT32-hCASP14-p20-C/A (LMBP 7890)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p7890.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human apoptosis-related cysteine peptidase 14 cDNA (CASP14, caspase 14, caspase-14, CASP-14); mutated p20 subunit
Promoter: Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Phage λ major leftward promoter (λ PL)
Ribosome
binding site:
-
Terminator: Phage T7 gene 10 terminator (T7g10)
Phage fd terminator
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains with a cI function, cIts for PL controlled expression
Parental clone: pLT32H
Further information: The plasmid contains a C132A mutant of the human CASP14 p20 subunit.
This low copy number plasmid can be used for IPTG-inducible expression of the mutated p20 subunit of human CASP14 after transformation into an E. coli strain containing a compatible piCA plasmid.
Expression of this p20 subunit of human CASP14 can be offered by the Protein Service Facility (PSF), Inflammation Research Center, VIB-Ghent University, Belgium. Please contact BCCM/GeneCorner for your protein expression request or refer to BCCM/GeneCorner when contacting PSF directly.
The nucleotide sequence of the wild type p20 subunit of human CASP14 corresponds with the EMBL Nucleotide Sequence Database accession number EU502748.1.
Other name of the plasmid is pLT32p20 C/A kloon1a.
EMBL Accession number: EU502748 .1, view at EMBL, GenBank, DDBJ
Latest sequence update: 24/06/2015
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr P. De Groote(1) (2) and Prof. Dr W. Declercq(1) (2).
(1) Inflammation Research Center, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Hoste et al., J. Invest. Dermatol. 131 (2011), 2233-2241 [PMID: 21654840]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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