Last data update: 15 January 2021 04:14 CET
Plasmid name: pLT32-hCASP14-p20 (LMBP 7891)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7891.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Human apoptosis-related cysteine peptidase 14 cDNA (CASP14, caspase 14, caspase-14, CASP-14); p20 subunit |
| Promoter: | Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) Phage fd terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pLT32H |
| Further information: | The plasmid contains the human CASP14 p20 subunit. This low copy number plasmid can be used for IPTG-inducible expression of the wild type p20 subunit of human CASP14 after transformation into an E. coli strain containing a compatible piCA plasmid. Expression of the p20 subunit of human CASP14 can be offered by the Protein Service Facility (PSF), Inflammation Research Center, VIB-Ghent University, Belgium. Please contact BCCM/GeneCorner for your protein expression request or refer to BCCM/GeneCorner when contacting PSF directly. The nucleotide sequence of the p20 subunit of human CASP14 corresponds with the EMBL Nucleotide Sequence Database accession number EU502748.1. Other name of the plasmid is pLT32p20 wt Kloon3b. |
| EMBL Accession number: | EU502748.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 24/06/2015 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. De Groote(1) (2) and Prof. Dr W. Declercq(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Hoste et al., J. Invest. Dermatol. 131 (2011), 2233-2241 [PMID: 21654840] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.